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Basics of Microscopy - First Steps in Neuroscience
Modern neuroscience increasingly relies on both basic and advanced microscopy techniques. However, when training is passed informally; from a peer, a supervisor, or someone in the lab; important knowledge is often lost or diluted over time.
This training, led by Dr Adrian Garcia‑Burgos (University of Edinburgh), provides a solid foundation in microscopy. As a microscopy application specialist for the IMPACT facility, Adrian has extensive experience training students with no prior knowledge and helping them become confident, advanced users.
This course is designed for anyone who wants more than just “button‑pushing” skills. You will learn the principles behind microscopy, the theory that guides good practice, and the hands‑on understanding needed to make informed decisions in your experiments.
Microscopy Journey:
- Light & fluorescence
- Sample preparation
- Brightfield, epifluorescence, confocal, and super‑resolution microscopy
- Image analysis
Topics Covered
- Nature of light: excitation/emission, refraction, chromatic aberrations, point spread function, and more
- Sample preparation: controls, experimental planning, good practices, photobleaching, mounting media
- Overview of microscopy techniques: benefits, limitations, and guidance on choosing the right system
- Fluorescent dyes: fundamentals and selection advice
- Image analysis tool available
Learning Outcomes
Participants will:
- Understand the core theoretical concepts used in microscopy
- Feel confident selecting the appropriate microscope for their sample
- Gain practical tips from an experienced specialist who trains over 150 users per year
Format
Online (Zoom) Dates: 2nd and 5th February 2026
Duration: Two half‑day sessions (3 hours of teaching per day, plus a break)
Time: 2-5PM
