This online workshop covers tissue clearing fundamentals and helps you choose the right method for various sample types.
Over the last decade, we have seen an ever-increasing number of clearing protocols, leaving scientists wondering which method will be suitable for their samples and provide answers to their research questions.
This workshop offers a unique opportunity to learn the fundamentals of tissue clearing and provides guidance on determining suitable approaches for clearing different types of samples.
Organiser: Dr Cristina Martinez Gonzalez
Instructors: Dr Cristina Martinez Gonzalez and Ms Kirsty Craigie.
Course content outline:
Learning outcomes for course attendees:
Participants can send questions in advance (during the registration process) regarding their tissue and experimental questions.
Senior Technical Officer, The University of Edinburgh
Kirsty is a senior scientist working at the Simons Initiative for the Developing Brain institute at the University of Edinburgh. Upon completing her postgraduate studies, Kirsty started at the University of Edinburgh as a Senior Technical Research Officer working on a project that aimed to explore brain-wide changes in rat models of autism spectrum disorders (ASD) using 3D imaging technologies.
Through this work, Kirsty became an expert in the field of optical tissue clearing and 3D light-sheet microscopy, and most recently co-developed RatDISCO - a novel immunolabelling and optical clearing pipeline that successfully labels markers of interest in large rat brains.
Currently, Kirsty works as a Senior Research Specialist in the 3D light-sheet microscopy imaging facility (LSM3D) at the Simons Initiative for the Developing Brain institute where she employs complex spatial technologies to answer a variety of neurological and therapeutic research questions. Beyond the lab, Kirsty is passionate about mentoring, teaching and supporting underrepresented scientific communities. In 2023, she received an award for being an outstanding mentor as part of the In2Science summer STEM programme, and in 2024, Kirsty obtained her Associate Fellowship teaching accreditation with the Higher Education Academy (Advance HE).
Research Fellow , University of Edinburgh
Dr Cristina Martinez Gonzalez is a Research Fellow at the University of Edinburgh and manager of the Light-Sheet Microscopy and 3D Image Analysis Facility. Her research focuses on mapping brain-wide circuit changes in neurological conditions, such as autism and epilepsy, using light-sheet microscopy and whole-brain clearing.
During her earlier postdoctoral work, Cristina contributed to breakthrough research in spinal cord injury. Her work on cortical–reticulospinal reorganization, driven by training and electrochemical stimulation, showed restored locomotion in rats after spinal cord injury. Using a viral method to reversibly silence gigantocellular neurons, she demonstrated that these brainstem neurons were essential for the regained movement—this work has since influenced human therapies.
Now, Cristina develops optical clearing methods like RatDISCO to immunolabel whole rat brains. She also established Scotland’s first open-access light-sheet microscopy facility, supporting interdisciplinary research on 3D brain structure and function. Beyond the lab, Cristina is passionate about mentoring, public engagement, and building inclusive scientific communities. In 2024, she received the Postdoc Citizenship Award for her leadership, advocacy, and support of early career researchers.
:
https://edwebprofiles.ed.ac.uk/profile/dr-cristina-martinez-gonzalez
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